Antibodies

See our next events!

CHO|360-HCP ELISA

The CHO|360-HCP ELISA is designed to cover a broader spectrum of Chinese hamster ovary (CHO) host cell proteins (HCPs) than traditional generic HCP assays.

How we made the antibodies

Polyclonal HCP antiserum was generated by immunizing rabbits and goats with HCP derived from mock transfected CHO-K1 and CHO-S cells combined with a unique approach for antigen and antibody preparation as well as an optimized purification strategy. We used differently prepared antigens: total HCP or fractionated HCP. Each HCP fraction was used individually for immunization resulting in four different HCP ELISA kits (type A to D) that together build up the enhanced generic CHO|360-HCP assay.

Superior HCP Recovery

The CHO|360-HCP ELISA has been widely tested on the basis of a great number of mock CHO-HCP samples. In most cases, the best recovery was estimated with one of our four generic CHO-HCP assays (type A to D). In the case of sampel 4(x), a recovery of >90% was estimated using assay type D. Recovery with assay types A to C was much lower, and recovery with the commercial assay was only 20%.

Product Specifications
Sensitivity CHO|360-HCP ELISA Kits (A–D)

  • LOD between 0.5 – 1.0 ng/mL
  • LOQ between 2 – 3 ng/mL
  • Working range 2 – 100 ng/mL

How it works

The Starter Set provides customers with all necessary components to fast and easily choose the most appropriate kit type (A, B, C or D). Alternatively, BioGenes conducts a Performance Study and recommends the optimal ELISA kit type as a service. For more information read here.

How to order

EU customers can place their order online or call +49 (0)30 65762396. Non-EU customers are requested to contact our distributors directly. If you have any questions, feel free to email us at order360@biogenes.de.

Contact

Dr. Yvonne Haberkorn
Product Manager & Head of Production Immunoassays
+49 (0)30 65762378