Antibodies

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E.coli|360-HCP ELISA

The E.coli|360-HCP ELISA is designed to cover a broader spectrum of E.coli host cell proteins (HCPs) than traditional generic HCP assays.

How we made the antibodies

E.coli|360-HCP Assay is based on W3110 and BL21(DE3) cell lines which were fermented in different culture media and conditions resulting in four antigen preparations with distinct HCP patterns. By immunizing goats with these antigens, BioGenes has developed a panel of four different HCP ELISAs (type A to D) that together build up the enhanced generic E.coli|360-HCP Assay. A high specificity for the antigen in all four assays was determined by Coomassie staining of the E.coli HCP antigen and 2D western blotting with the respective antibodies.

Superior HCP Recovery

E.coli|360-HCP Assay has been widely tested on the basis of a great number of mock E.coli HCP samples. In case of sample 1 a recovery of 80% was estimated using assay type B. For sample 2 and 3 the best recovery rate was determined using kit type A. For sample 5 a sufficient recovery was only determined using kit type D, recovery rates determined with the other three kit types were below 30%.

Product Specifications
Sensitivity e.coli|360-HCP Kits (A–D)

  • LOD between 0.2 – 0.5 ng/mL
  • LOQ between 0.6 – 1.6 ng/mL
  • Working range 2 – 100 ng/mL

How it works

The Starter Set provides customers with all necessary components to fast and easily choose the most appropriate kit type (A, B, C or D). Alternatively, BioGenes conducts a Performance Study and recommends the optimal ELISA kit type as a service. For more information read here.

How to order

EU customers can place their order online or call +49 (0)30 65762396. Non-EU customers are requested to contact our distributors directly. If you have any questions, feel free to email us at order360@biogenes.de.

Contact

Dr. Yvonne Haberkorn
Product Manager & Head of Production Immunoassays
+49 (0)30 65762378