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Performance Study

The 360-HCP ELISAs require the identification of the most suitable kit type (A, B, C or D) for a specific process. The Starter Set provides customers with all necessary components to fast and easily choose the most appropriate kit. Alternatively, BioGenes conducts a Performance Study and recommends the optimal ELISA kit type as a service.

Performance Study: Part 1

Characterisation of HCP-containing samples using 360-HCP ELISA kits (types A to D):

  • Customer provides 3 in-process controls (IPCs) from different purification steps
  • Determination of the sample’s HCP content using all four BioGenes 360-HCP ELISA kits
  • Preparation of 1D Western Blots of the samples followed by immunostaining with respective antibody from BioGenes 360-HCP ELISA kits
  • Testing of the accuracy by spiking experiments
  • BioGenes recommends the most suitable ELISA kit type (A to D) including short report

Performance Study: Part 2

2D Analysis (coverage determination) using the selected antibody (type A, B, C or D):

2D analysis allows for estimation of anti-HCP antibody’s coverage of the total HCPs present in a cell line. The aim of part 2 is to confirm the suitability of the antibody (A to D) chosen in part 1. BioGenes offers 2D analysis of a process sample using the anti-HCP antibody selected before.

  • 2D separation of the samples (fluorescence-based)
  • Immunoblot using the selected antibodies
  • Detailed report including estimation of HCP coverage


Dr. Yvonne Haberkorn
Product Manager & Head of Production Immunoassays
+49 (0)30 65762378